stem pro adipocyte differentiation basal medium supplement Search Results


stem pro adipocyte differentiation media  (Thermo Fisher)
86
Thermo Fisher stem pro adipocyte differentiation media
UVA irradiation inhibits adipocyte differentiation. Two-day post-confluent hAMSCs (day 0) were treated with the indicated doses of UVA irradiation and then stimulated with <t>STEM</t> <t>PRO®</t> adipocyte differentiation (DIF) media for 3 days. The medium was then replaced with STEM PRO® adipocyte differentiation media every 3 days until the end of the experiment at day 14. The assays were performed on fully differentiated adipocytes (day 14). A, intracellular lipids were stained with Oil Red O. The results were confirmed by three independent experiments, which were each conducted in duplicate. B, triglyceride content was measured using a triglyceride assay kit (Cayman Chemical, Ann Arbor, MI). Data are expressed as the means ± S.D. *, p < 0.05 versus controls. The results were verified by three repetitions of the experiments, each of which was conducted in triplicate. C, GPDH activity was measured using GPDH activity assay kits (Takara Bio Inc., Japan). Data are expressed as the means ± S.D. *, p < 0.05 versus controls. The results were verified by four repetitions of the experiments. D, 2-day post-confluent 3T3-L1 preadipocytes (day 0) were treated with the indicated concentrations of UVA irradiation and then stimulated with differentiation medium for 3 days. The medium was then replaced with maintenance media every 3 days until the end of the experiment on day 9. The assays were performed on fully differentiated adipocytes (day 9). Intracellular lipids were stained with Oil Red O. The results were confirmed by three independent experiments, which were each conducted in duplicate. E, general viability of cultured cells was determined by the reduction of WST-8 to a highly water-soluble formazan dye. The results were verified by four repetitions of the experiments. F, DNA-damaging effects of UVA irradiation were determined using a DNA Damage ELISA kit. The results were verified by four repetitions of the experiments. G, apoptotic effects of UVA irradiation were determined by Hoechst 33332 staining. The results were verified by four repetitions of the experiments. DIF/MDI, differentiation media.
Stem Pro Adipocyte Differentiation Media, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/stem pro adipocyte differentiation media/product/Thermo Fisher
Average 86 stars, based on 1 article reviews
stem pro adipocyte differentiation media - by Bioz Stars, 2026-03
86/100 stars
  Buy from Supplier
stem pro adipocyte differentiation basal medium supplement  (Thermo Fisher)
90
Thermo Fisher stem pro adipocyte differentiation basal medium supplement
UVA irradiation inhibits adipocyte differentiation. Two-day post-confluent hAMSCs (day 0) were treated with the indicated doses of UVA irradiation and then stimulated with <t>STEM</t> <t>PRO®</t> adipocyte differentiation (DIF) media for 3 days. The medium was then replaced with STEM PRO® adipocyte differentiation media every 3 days until the end of the experiment at day 14. The assays were performed on fully differentiated adipocytes (day 14). A, intracellular lipids were stained with Oil Red O. The results were confirmed by three independent experiments, which were each conducted in duplicate. B, triglyceride content was measured using a triglyceride assay kit (Cayman Chemical, Ann Arbor, MI). Data are expressed as the means ± S.D. *, p < 0.05 versus controls. The results were verified by three repetitions of the experiments, each of which was conducted in triplicate. C, GPDH activity was measured using GPDH activity assay kits (Takara Bio Inc., Japan). Data are expressed as the means ± S.D. *, p < 0.05 versus controls. The results were verified by four repetitions of the experiments. D, 2-day post-confluent 3T3-L1 preadipocytes (day 0) were treated with the indicated concentrations of UVA irradiation and then stimulated with differentiation medium for 3 days. The medium was then replaced with maintenance media every 3 days until the end of the experiment on day 9. The assays were performed on fully differentiated adipocytes (day 9). Intracellular lipids were stained with Oil Red O. The results were confirmed by three independent experiments, which were each conducted in duplicate. E, general viability of cultured cells was determined by the reduction of WST-8 to a highly water-soluble formazan dye. The results were verified by four repetitions of the experiments. F, DNA-damaging effects of UVA irradiation were determined using a DNA Damage ELISA kit. The results were verified by four repetitions of the experiments. G, apoptotic effects of UVA irradiation were determined by Hoechst 33332 staining. The results were verified by four repetitions of the experiments. DIF/MDI, differentiation media.
Stem Pro Adipocyte Differentiation Basal Medium Supplement, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/stem pro adipocyte differentiation basal medium supplement/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
stem pro adipocyte differentiation basal medium supplement - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
adipocytes differentiation medium  (Millipore)
90
Millipore adipocytes differentiation medium
UVA irradiation inhibits adipocyte differentiation. Two-day post-confluent hAMSCs (day 0) were treated with the indicated doses of UVA irradiation and then stimulated with <t>STEM</t> <t>PRO®</t> adipocyte differentiation (DIF) media for 3 days. The medium was then replaced with STEM PRO® adipocyte differentiation media every 3 days until the end of the experiment at day 14. The assays were performed on fully differentiated adipocytes (day 14). A, intracellular lipids were stained with Oil Red O. The results were confirmed by three independent experiments, which were each conducted in duplicate. B, triglyceride content was measured using a triglyceride assay kit (Cayman Chemical, Ann Arbor, MI). Data are expressed as the means ± S.D. *, p < 0.05 versus controls. The results were verified by three repetitions of the experiments, each of which was conducted in triplicate. C, GPDH activity was measured using GPDH activity assay kits (Takara Bio Inc., Japan). Data are expressed as the means ± S.D. *, p < 0.05 versus controls. The results were verified by four repetitions of the experiments. D, 2-day post-confluent 3T3-L1 preadipocytes (day 0) were treated with the indicated concentrations of UVA irradiation and then stimulated with differentiation medium for 3 days. The medium was then replaced with maintenance media every 3 days until the end of the experiment on day 9. The assays were performed on fully differentiated adipocytes (day 9). Intracellular lipids were stained with Oil Red O. The results were confirmed by three independent experiments, which were each conducted in duplicate. E, general viability of cultured cells was determined by the reduction of WST-8 to a highly water-soluble formazan dye. The results were verified by four repetitions of the experiments. F, DNA-damaging effects of UVA irradiation were determined using a DNA Damage ELISA kit. The results were verified by four repetitions of the experiments. G, apoptotic effects of UVA irradiation were determined by Hoechst 33332 staining. The results were verified by four repetitions of the experiments. DIF/MDI, differentiation media.
Adipocytes Differentiation Medium, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/adipocytes differentiation medium/product/Millipore
Average 90 stars, based on 1 article reviews
adipocytes differentiation medium - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
stem pro® adipogenesis differentiation medium  (Thermo Fisher)
90
Thermo Fisher stem pro® adipogenesis differentiation medium
UVA irradiation inhibits adipocyte differentiation. Two-day post-confluent hAMSCs (day 0) were treated with the indicated doses of UVA irradiation and then stimulated with <t>STEM</t> <t>PRO®</t> adipocyte differentiation (DIF) media for 3 days. The medium was then replaced with STEM PRO® adipocyte differentiation media every 3 days until the end of the experiment at day 14. The assays were performed on fully differentiated adipocytes (day 14). A, intracellular lipids were stained with Oil Red O. The results were confirmed by three independent experiments, which were each conducted in duplicate. B, triglyceride content was measured using a triglyceride assay kit (Cayman Chemical, Ann Arbor, MI). Data are expressed as the means ± S.D. *, p < 0.05 versus controls. The results were verified by three repetitions of the experiments, each of which was conducted in triplicate. C, GPDH activity was measured using GPDH activity assay kits (Takara Bio Inc., Japan). Data are expressed as the means ± S.D. *, p < 0.05 versus controls. The results were verified by four repetitions of the experiments. D, 2-day post-confluent 3T3-L1 preadipocytes (day 0) were treated with the indicated concentrations of UVA irradiation and then stimulated with differentiation medium for 3 days. The medium was then replaced with maintenance media every 3 days until the end of the experiment on day 9. The assays were performed on fully differentiated adipocytes (day 9). Intracellular lipids were stained with Oil Red O. The results were confirmed by three independent experiments, which were each conducted in duplicate. E, general viability of cultured cells was determined by the reduction of WST-8 to a highly water-soluble formazan dye. The results were verified by four repetitions of the experiments. F, DNA-damaging effects of UVA irradiation were determined using a DNA Damage ELISA kit. The results were verified by four repetitions of the experiments. G, apoptotic effects of UVA irradiation were determined by Hoechst 33332 staining. The results were verified by four repetitions of the experiments. DIF/MDI, differentiation media.
Stem Pro® Adipogenesis Differentiation Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/stem pro® adipogenesis differentiation medium/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
stem pro® adipogenesis differentiation medium - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
stem pro adipocyte differentiation medium  (Thermo Fisher)
90
Thermo Fisher stem pro adipocyte differentiation medium
UVA irradiation inhibits adipocyte differentiation. Two-day post-confluent hAMSCs (day 0) were treated with the indicated doses of UVA irradiation and then stimulated with <t>STEM</t> <t>PRO®</t> adipocyte differentiation (DIF) media for 3 days. The medium was then replaced with STEM PRO® adipocyte differentiation media every 3 days until the end of the experiment at day 14. The assays were performed on fully differentiated adipocytes (day 14). A, intracellular lipids were stained with Oil Red O. The results were confirmed by three independent experiments, which were each conducted in duplicate. B, triglyceride content was measured using a triglyceride assay kit (Cayman Chemical, Ann Arbor, MI). Data are expressed as the means ± S.D. *, p < 0.05 versus controls. The results were verified by three repetitions of the experiments, each of which was conducted in triplicate. C, GPDH activity was measured using GPDH activity assay kits (Takara Bio Inc., Japan). Data are expressed as the means ± S.D. *, p < 0.05 versus controls. The results were verified by four repetitions of the experiments. D, 2-day post-confluent 3T3-L1 preadipocytes (day 0) were treated with the indicated concentrations of UVA irradiation and then stimulated with differentiation medium for 3 days. The medium was then replaced with maintenance media every 3 days until the end of the experiment on day 9. The assays were performed on fully differentiated adipocytes (day 9). Intracellular lipids were stained with Oil Red O. The results were confirmed by three independent experiments, which were each conducted in duplicate. E, general viability of cultured cells was determined by the reduction of WST-8 to a highly water-soluble formazan dye. The results were verified by four repetitions of the experiments. F, DNA-damaging effects of UVA irradiation were determined using a DNA Damage ELISA kit. The results were verified by four repetitions of the experiments. G, apoptotic effects of UVA irradiation were determined by Hoechst 33332 staining. The results were verified by four repetitions of the experiments. DIF/MDI, differentiation media.
Stem Pro Adipocyte Differentiation Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/stem pro adipocyte differentiation medium/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
stem pro adipocyte differentiation medium - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
rat sterol regulatory element-binding protein 1c  (Shanghai Korain Biotech Co Ltd)
N/A
Precursor of the transcription factor form (Processed sterol regulatory element-binding protein 1), which is embedded in the endoplasmic reticulum membrane (By similarity). Low sterol concentrations promote processing of this form, releasing the transcription factor form
   Buy from Supplier
fatty acid binding protein adipocyte western blot control protein oprb00226  (Aviva Systems)
N/A
Fatty acid binding proteins are a family of small highly conserved cytoplasmic proteins 14 15kDa that bind long chain fatty acids their CoA derivatives billiruben organic anions and other small hydrophobic molecules A FABP or
   Buy from Supplier
rat sterol regulatory element-binding protein 1  (Shanghai Korain Biotech Co Ltd)
N/A
[Sterol regulatory element-binding protein 1]: Precursor of the transcription factor form (Processed sterol regulatory element-binding protein 1), which is embedded in the endoplasmic reticulum membrane (By similarity). Low sterol concentrations promote processing of this form,
   Buy from Supplier

Image Search Results


UVA irradiation inhibits adipocyte differentiation. Two-day post-confluent hAMSCs (day 0) were treated with the indicated doses of UVA irradiation and then stimulated with STEM PRO® adipocyte differentiation (DIF) media for 3 days. The medium was then replaced with STEM PRO® adipocyte differentiation media every 3 days until the end of the experiment at day 14. The assays were performed on fully differentiated adipocytes (day 14). A, intracellular lipids were stained with Oil Red O. The results were confirmed by three independent experiments, which were each conducted in duplicate. B, triglyceride content was measured using a triglyceride assay kit (Cayman Chemical, Ann Arbor, MI). Data are expressed as the means ± S.D. *, p < 0.05 versus controls. The results were verified by three repetitions of the experiments, each of which was conducted in triplicate. C, GPDH activity was measured using GPDH activity assay kits (Takara Bio Inc., Japan). Data are expressed as the means ± S.D. *, p < 0.05 versus controls. The results were verified by four repetitions of the experiments. D, 2-day post-confluent 3T3-L1 preadipocytes (day 0) were treated with the indicated concentrations of UVA irradiation and then stimulated with differentiation medium for 3 days. The medium was then replaced with maintenance media every 3 days until the end of the experiment on day 9. The assays were performed on fully differentiated adipocytes (day 9). Intracellular lipids were stained with Oil Red O. The results were confirmed by three independent experiments, which were each conducted in duplicate. E, general viability of cultured cells was determined by the reduction of WST-8 to a highly water-soluble formazan dye. The results were verified by four repetitions of the experiments. F, DNA-damaging effects of UVA irradiation were determined using a DNA Damage ELISA kit. The results were verified by four repetitions of the experiments. G, apoptotic effects of UVA irradiation were determined by Hoechst 33332 staining. The results were verified by four repetitions of the experiments. DIF/MDI, differentiation media.

Journal: The Journal of Biological Chemistry

Article Title: Ultraviolet A Regulates Adipogenic Differentiation of Human Adipose Tissue-derived Mesenchymal Stem Cells via Up-regulation of Kruppel-like Factor 2

doi: 10.1074/jbc.M110.135830

Figure Lengend Snippet: UVA irradiation inhibits adipocyte differentiation. Two-day post-confluent hAMSCs (day 0) were treated with the indicated doses of UVA irradiation and then stimulated with STEM PRO® adipocyte differentiation (DIF) media for 3 days. The medium was then replaced with STEM PRO® adipocyte differentiation media every 3 days until the end of the experiment at day 14. The assays were performed on fully differentiated adipocytes (day 14). A, intracellular lipids were stained with Oil Red O. The results were confirmed by three independent experiments, which were each conducted in duplicate. B, triglyceride content was measured using a triglyceride assay kit (Cayman Chemical, Ann Arbor, MI). Data are expressed as the means ± S.D. *, p < 0.05 versus controls. The results were verified by three repetitions of the experiments, each of which was conducted in triplicate. C, GPDH activity was measured using GPDH activity assay kits (Takara Bio Inc., Japan). Data are expressed as the means ± S.D. *, p < 0.05 versus controls. The results were verified by four repetitions of the experiments. D, 2-day post-confluent 3T3-L1 preadipocytes (day 0) were treated with the indicated concentrations of UVA irradiation and then stimulated with differentiation medium for 3 days. The medium was then replaced with maintenance media every 3 days until the end of the experiment on day 9. The assays were performed on fully differentiated adipocytes (day 9). Intracellular lipids were stained with Oil Red O. The results were confirmed by three independent experiments, which were each conducted in duplicate. E, general viability of cultured cells was determined by the reduction of WST-8 to a highly water-soluble formazan dye. The results were verified by four repetitions of the experiments. F, DNA-damaging effects of UVA irradiation were determined using a DNA Damage ELISA kit. The results were verified by four repetitions of the experiments. G, apoptotic effects of UVA irradiation were determined by Hoechst 33332 staining. The results were verified by four repetitions of the experiments. DIF/MDI, differentiation media.

Article Snippet: Immunoblotting Two-day post-confluent hAMSCs were irradiated with the indicated doses of UVA and then stimulated with STEM PRO® adipocyte differentiation media (Invitrogen) for 3 days.

Techniques: Irradiation, Staining, Activity Assay, Cell Culture, Enzyme-linked Immunosorbent Assay

Effects of UVA irradiation on the expression of the adipogenic transcription factors. hAMSCs (day 0) were treated with the indicated doses of UVA irradiation and then stimulated with STEM PRO® adipocyte differentiation media for 3 days. The medium was then replaced with STEM PRO® adipocyte differentiation media every 3 days until the end of the experiment on day 14. A and D--G, at 1 day or 14 days after the induction of differentiation, the total RNA was isolated, and the mRNA levels of the PPAR γ (A), C/EBP α (D), C/EBP β and C/EBP δ (E), aP2 and LPL (F), and CD36 and LXR α and (G) genes were measured by real time quantitative RT-PCR. The results are expressed relative to untreated cells after normalization against the 18 S rRNA. Data are expressed as the means ± S.D. *, p < 0.05 versus controls. The results were verified by four repetitions of the experiments, each of which was conducted in triplicate. B, at day 14 after the induction of differentiation, the cell lysates were analyzed by Western blot analysis using the indicated antibodies. The results were verified by three repetitions of the experiments, each of which was conducted in duplicate. C, hAMSCs were cotransfected with luciferase constructs that contained PPRE×3-Luc reporter and PPAR γ cDNA plasmid. After 16 h, the transfected cells were incubated for 20 h with the indicated doses of UVA irradiation. The cells were then harvested and lysed. Luciferase activity is expressed as the ratio of PPRE×3 promoter-dependent firefly luciferase activity divided by control thymidine kinase Renilla luciferase activity (relative luciferase units). Data are expressed as the means ± S.D. *, p < 0.05 versus controls. The experiments were repeated eight times, with each experiment being conducted in triplicate. DIF, differentiation media.

Journal: The Journal of Biological Chemistry

Article Title: Ultraviolet A Regulates Adipogenic Differentiation of Human Adipose Tissue-derived Mesenchymal Stem Cells via Up-regulation of Kruppel-like Factor 2

doi: 10.1074/jbc.M110.135830

Figure Lengend Snippet: Effects of UVA irradiation on the expression of the adipogenic transcription factors. hAMSCs (day 0) were treated with the indicated doses of UVA irradiation and then stimulated with STEM PRO® adipocyte differentiation media for 3 days. The medium was then replaced with STEM PRO® adipocyte differentiation media every 3 days until the end of the experiment on day 14. A and D--G, at 1 day or 14 days after the induction of differentiation, the total RNA was isolated, and the mRNA levels of the PPAR γ (A), C/EBP α (D), C/EBP β and C/EBP δ (E), aP2 and LPL (F), and CD36 and LXR α and (G) genes were measured by real time quantitative RT-PCR. The results are expressed relative to untreated cells after normalization against the 18 S rRNA. Data are expressed as the means ± S.D. *, p < 0.05 versus controls. The results were verified by four repetitions of the experiments, each of which was conducted in triplicate. B, at day 14 after the induction of differentiation, the cell lysates were analyzed by Western blot analysis using the indicated antibodies. The results were verified by three repetitions of the experiments, each of which was conducted in duplicate. C, hAMSCs were cotransfected with luciferase constructs that contained PPRE×3-Luc reporter and PPAR γ cDNA plasmid. After 16 h, the transfected cells were incubated for 20 h with the indicated doses of UVA irradiation. The cells were then harvested and lysed. Luciferase activity is expressed as the ratio of PPRE×3 promoter-dependent firefly luciferase activity divided by control thymidine kinase Renilla luciferase activity (relative luciferase units). Data are expressed as the means ± S.D. *, p < 0.05 versus controls. The experiments were repeated eight times, with each experiment being conducted in triplicate. DIF, differentiation media.

Article Snippet: Immunoblotting Two-day post-confluent hAMSCs were irradiated with the indicated doses of UVA and then stimulated with STEM PRO® adipocyte differentiation media (Invitrogen) for 3 days.

Techniques: Irradiation, Expressing, Isolation, Quantitative RT-PCR, Western Blot, Luciferase, Construct, Plasmid Preparation, Transfection, Incubation, Activity Assay

Effects of UVA irradiation on the production of anti-adipogenic cytokines. hAMSCs (day 0) were treated with the indicated doses of UVA irradiation and then stimulated with STEM PRO® adipocyte differentiation media for 3 days. The medium was then replaced with STEM PRO® adipocyte differentiation media every 3 days until the end of the experiment on day 14. At day 14 after the induction of differentiation, the supernatants were harvested for TNF-α (A), IL-1β (B), VEGF (C), TGF-β1 (D), and MIF (E and G) measurements. Data are expressed as the means ± S.D. *, p < 0.05 versus controls. The results were verified by three repetitions of the experiments, each of which was conducted in duplicate. F, at 14 days after the induction of differentiation, the total RNA was isolated, and the mRNA levels of the PPAR γ gene were measured by real time quantitative RT-PCR. The results are expressed relative to untreated cells after normalization against 18 S rRNA. Data are expressed as the means ± S.D. *, p < 0.05 versus controls. The results were verified by four repetitions of the experiments, each of which was conducted in triplicate. DMI, differentiation media; PD, PD98059 (a p42/44 MAPK inhibitor); SB, SB203580 (a p38 MAPK inhibitor); SP, SP600125 (JNK inhibitor); PDTC, pyrrolidine dithiocarbamate.

Journal: The Journal of Biological Chemistry

Article Title: Ultraviolet A Regulates Adipogenic Differentiation of Human Adipose Tissue-derived Mesenchymal Stem Cells via Up-regulation of Kruppel-like Factor 2

doi: 10.1074/jbc.M110.135830

Figure Lengend Snippet: Effects of UVA irradiation on the production of anti-adipogenic cytokines. hAMSCs (day 0) were treated with the indicated doses of UVA irradiation and then stimulated with STEM PRO® adipocyte differentiation media for 3 days. The medium was then replaced with STEM PRO® adipocyte differentiation media every 3 days until the end of the experiment on day 14. At day 14 after the induction of differentiation, the supernatants were harvested for TNF-α (A), IL-1β (B), VEGF (C), TGF-β1 (D), and MIF (E and G) measurements. Data are expressed as the means ± S.D. *, p < 0.05 versus controls. The results were verified by three repetitions of the experiments, each of which was conducted in duplicate. F, at 14 days after the induction of differentiation, the total RNA was isolated, and the mRNA levels of the PPAR γ gene were measured by real time quantitative RT-PCR. The results are expressed relative to untreated cells after normalization against 18 S rRNA. Data are expressed as the means ± S.D. *, p < 0.05 versus controls. The results were verified by four repetitions of the experiments, each of which was conducted in triplicate. DMI, differentiation media; PD, PD98059 (a p42/44 MAPK inhibitor); SB, SB203580 (a p38 MAPK inhibitor); SP, SP600125 (JNK inhibitor); PDTC, pyrrolidine dithiocarbamate.

Article Snippet: Immunoblotting Two-day post-confluent hAMSCs were irradiated with the indicated doses of UVA and then stimulated with STEM PRO® adipocyte differentiation media (Invitrogen) for 3 days.

Techniques: Irradiation, Isolation, Quantitative RT-PCR

UVA irradiation has no effects on osteoblast differentiation in hAMSCs. Two-day post-confluent hAMSCs (day 0) were treated with the indicated doses of UVA irradiation and then stimulated with STEM PRO® osteogenesis differentiation media for 3 days. The medium was then replaced with STEM PRO® osteogenesis differentiation media every 3 days until the end of the experiment at day 14. A, alkaline phosphatase staining. Osteogenesis was confirmed with alkaline phosphatase staining. The results were confirmed by three independent experiments, which were each conducted in duplicate. B, alkaline phosphatase activity was measured in cell lysates for 14 days, using a colorimetric assay, and then normalized to the level of total cellular protein. Data are expressed as the means ± S.D. *, p < 0.05 versus controls. The results were confirmed by three independent experiments, which were each conducted in duplicate. ODIF, osteogenic differentiation media.

Journal: The Journal of Biological Chemistry

Article Title: Ultraviolet A Regulates Adipogenic Differentiation of Human Adipose Tissue-derived Mesenchymal Stem Cells via Up-regulation of Kruppel-like Factor 2

doi: 10.1074/jbc.M110.135830

Figure Lengend Snippet: UVA irradiation has no effects on osteoblast differentiation in hAMSCs. Two-day post-confluent hAMSCs (day 0) were treated with the indicated doses of UVA irradiation and then stimulated with STEM PRO® osteogenesis differentiation media for 3 days. The medium was then replaced with STEM PRO® osteogenesis differentiation media every 3 days until the end of the experiment at day 14. A, alkaline phosphatase staining. Osteogenesis was confirmed with alkaline phosphatase staining. The results were confirmed by three independent experiments, which were each conducted in duplicate. B, alkaline phosphatase activity was measured in cell lysates for 14 days, using a colorimetric assay, and then normalized to the level of total cellular protein. Data are expressed as the means ± S.D. *, p < 0.05 versus controls. The results were confirmed by three independent experiments, which were each conducted in duplicate. ODIF, osteogenic differentiation media.

Article Snippet: Immunoblotting Two-day post-confluent hAMSCs were irradiated with the indicated doses of UVA and then stimulated with STEM PRO® adipocyte differentiation media (Invitrogen) for 3 days.

Techniques: Irradiation, Staining, Activity Assay, Colorimetric Assay